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pRSVhPit-1v
pRSVhPit-1v
規(guī)格:
貨期:
編號:B244938
品牌:Mingzhoubio

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產(chǎn)品名稱 pRSVhPit-1v
商品貨號 B244938
Designations pRSVhPit-1v
GenBank Number

X72215

Species Homo sapiens, human
Applications
in another host, produces protein pituitary-specific transcription factor (growth hormone factor 1)
Vector
Construct size (kb): 5.599999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pRSVcat
Intact vector size: 5.100
Type of vector: plasmid
Vector end: XmaI
Vector end: XbaI
Cloning sites: BamHI HindIII
Polylinker sites:
Construction: pSV2-betaG, Tn9, RSV
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: RSV
marker(s): ampR, cmLR
promoter: RSV LTR
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PIT1
Genomic copy number: unique
Gene name: pituitary-specific transcription factor (growth hormone
factor 1)
Contains complete coding sequence?: Y
Chromosome: 3; Localization: 3 p11
Tissue: pituitary
Type of DNA: cDNA
Insert 5' end: XmaI
Insert 3' end: XbaI
Insert size (kb): 1.0
Cross references: DNA Seq. Acc.: X72215
Insert lengths(kb): 1.0
Tissue: pituitary
Gene product: pituitary-specific transcription factor (growth hormone factor 1) [PIT1]
Target Gene: pituitary-specific transcription factor (growth hormone factor 1)
Insert Size (kb) 1.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.1, 0.4, 0.24; PstI--2.5, 1.8, 1.0; PvuII--5.4; SphI--4.6, 0.9; SmaI/XbaI--4.4, 1.1.
Insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.11, 0.56; PstI--0.6; PvuII--0.38, 0.54; SphI--0.62.
Contains the complete coding sequence plus portions of the 5' and 3' untranslated regions.
This clone varies from other published sequence(s): GenBank records X62429, D01114. The aa sequence differences determined by GenBank X72215 are: Q4R, D227Y.
The ends of the PIT1-specific portions of the primers used to amplify this sequence correspond to nt 56-1046 of GenBank record D01114. In this record, the coding region extends from nt 126-1001.
Insert was amplified using the following primers: 5'CCCCCCGGGAGACAGTAATATAATAA 3'(5' untranslated region) and 5'CCCTCTAGAGAAGGAATGAAACGGG 3' (3' untranslated region).
References

Pernasetti F, et al. Cloning of a human GHF-1/Pit-1 cDNA variant. Nucleic Acids Res. 21: 3584, 1993. PubMed: 8346040

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