Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.2, 2.7, 1.5, 1.0; EcoRI--9.0, 2.7; HindIII--6.2, 2.7, 1.5, 1.0. Use probe prehybridization; the insert may be single copy. The HindIII and BglII polymorphisms are apparently in complete disequilibrium. The signal is clean with both enzyme digests. |