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pMTK4
pMTK4
規(guī)格:
貨期:
編號(hào):B243754
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pMTK4
商品貨號(hào) B243754
Designations pMTK4
GenBank Number

M11945

Species Mus musculus, mouse
Depositors FH Ruddle
Vector
Construct size (kb): 4.360000133514404
Insert
DNA: cDNA
Insert lengths(kb): 1.1599999666214
Tissue: L cell
Gene product: thymidine kinase 1, soluble [Tk1]
Insert Size (kb) 1.160
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Insert contains MspI (2), Sau3AI (2), TaqI, HaeIII (7), ClaI, and AvaI (3) sites (not including the polylinker).
Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 1.5; SalI--4.7; PstI/HindIII--3.2, 0.86, 0.5.
Contains a functional cDNA for cytoplasmic thymidine kinase which is close to full length.
pMTK4 detects genomic HindIII fragments of 23 kb and 4.7 kb in C57BL/10J, C3H/HeJ, A/HeJ, and BALB/cJ mice. It also detects 9.3 kb and 3.7 kb fragments also present in Ltk- cells, suggesting the latter are not functional cytosolic Tk genes.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Lin PF, et al. Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences. Mol. Cell. Biol. 5: 3149-3156, 1985. PubMed: 3018504

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