亚洲日韩av无码,国产精品成人无码久久久久久,久久久久人妻精品一区三寸蜜桃,日韩精品内射视频免费观看,国产精品无码AV无码,人妻丰满熟妇av无码区免,久久夜色撩人精品国产小说,无码AV免费一区二区三区试看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > Vaccinia virus ts mutant
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Vaccinia virus ts mutant
Vaccinia virus ts mutant
規(guī)格:
貨期:
編號(hào):B243164
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Vaccinia virus ts mutant
商品貨號(hào) B243164
Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts47 (Dales isolate 6828)
Applications
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U12.
Positive for Mycoplasma No
Biosafety Level 2 CDC BMBL recommends vaccination.
Isolation
derived from existing strain
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned a Dales EM category of P, characterized by immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U12.
Effect on Host
Yes, Cytopathic effect: Rounding and sloughing of infected cells
CPE: enlargement, plaques with rounding and eventual sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host: BSC-40 (ATCC? CRL-2761
Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 31.0°C
Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent [not 100% confluent] for inoculation.
Cryopreservation
Storage temperature: -70°C
Name of Depositor S Dales, RC Condit, S Dales
Special Collection ATCC
Source
derived from existing strain
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain.

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Vaccination recommended for laboratory staff manipulating vaccinia, monkeypox, or cowpox viruses [CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4].

or colder

Non-permissive incubation temperature is 39.5C. This mutant was assigned a Dales EM category of P. It is assigned the Condit map location U12. This subtype is distinguishable from the parent IHD-J strain by its ability to elicit polykaryocytosis in all mammalian and avian cells tested and by its inability to induce an active hemagglutinin at the plasma membrane.

or colder

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
略阳县| 于田县| 顺昌县| 合阳县| 江华| 阿拉善盟| 墨竹工卡县| 衢州市| 高州市| 重庆市| 平利县| 黄平县| 贺州市| 娱乐| 桦甸市| 宣城市| 论坛| 肇庆市| 镇平县| 博爱县| 定陶县| 满洲里市| 田林县| 开鲁县| 福安市| 龙泉市| 连平县| 韶山市| 邢台县| 昌邑市| 遵义县| 光泽县| 开远市| 门头沟区| 固镇县| 顺昌县| 吉林市| 杂多县| 普定县| 临泉县| 张家川|