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Escherichia coli HB101 (ATCC 33694)

Intact vector size: 9.300

Type of vector: plasmid
Cloning sites: XhoI SalI BamHI SmaI
Polylinker sites: XhoI SalI BamHI SmaI
Cloning capacity: Y
Construction: REP4X
Host range: Schizosaccharomyces pombe; Escherichia coli
Features (with orientation and position when available):
coding sequence: GFP, ->
replicon: ars1
marker(s): ampR
marker(s): ura4+
promoter for expression: nmt, ->
MCS: XhoI...SmaI, ->

Vector: pSGP573 (plasmid)

Promoters: Promoter for expression nmt

Construction: REP4X

Marker(s): ampR

Construct size (kb): 9.30

Features: marker(s): ampR
marker(s): ura4+
promoter for expression: nmt
replicon: ars1
MCS: XhoI...SmaI
coding sequence: GFP

contains sequence green fluorescent protein (GFP)

GFP sequence corresponds to Genbank Accession number U87624 with nucleotides 7-720 of GFP matching nucleotides 21-734 of U87624, with additional modification S65T by changing codon to ACC.

The order of the major features in this plasmid is: nmt promotor - XhoI - ATG - GFP - GCT - NotI - SalI - BglII - SmaI - termination sequence - ars1 - pUC119 (3.3 kb) - ura4

Temperature: 37°C