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Distribution host: Escherichia coli JS219

Size (kb): 6.0000000000000000

DESCRIPTION OF VECTOR:
Intact vector size: 6.000
Type of vector: plasmid
Cloning sites: BamHI SalI PstI SphI XmaIII HindIII EcoRI SacI KpnI
Polylinker sites: BamHI SalI PstI SphI HindIII EcoRI SacI KpnI BamHI
Construction: pBR322, R100.1, pCK1
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: T4gene32 ter
marker(s): ampR, spcR
replicon: pMB1
repressor gene: lacIq

Vector: pAM34 (plasmid)

Construction: pBR322, R100.1, pCK1

Marker(s):ampR,spcR

Construct size (kb): 6.0

Features: insert detection: T4gene32 ter
marker(s): ampR, spcR
replicon: pMB1
repressor gene: lacIq

integrating vector

vector permitting positive selection for inserts

Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; BamHI--4.3, 1.8; HindIII--6.0; PstI--6.0; KpnI--3.4, 2.7.

Can be selected with 100 ug/ml ampicillin and/or 75 ug/ml spectinomycin. Permits positive selection because deletion of the aadA fragment is not viable.

E. coli JS219 is MC1061 malPp delta534::lacIq.

Integration vector permitting positive selection for inserts.

Transcription of the replication primer RNA is regulated by the lacZpo promoter/operator. In the presence of lacIq, replication is entirely dependent on the presence of inducer.

The repressible replication system permits temporary maintenance of the plasmid, construction of strains with stable integrants of vector derivatives, and recovery of sequences adjacent to cloned fragments.

pAM35 (ATCC 77186) differs from pAM34 (ATCC 77185) by deletion of the DraII fragment in pAM34 carrying lacIq.

The order of the major features in this plasmid is: lacZpo - ori - rom - transcription terminator - BamHI - MCS - XmaIII - aadA (spcR) - MCS - BamHI - transcription terminator - bla - lacIq.

Temperature: 37.0°C