PstI cloning site distal to pre-penicillinase signal sequence.
Vector for secretion of cloned gene product.
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References
Talmadge K, Gilberg W. Construction of plasmid vectors with unique PstI cloning sites in a signal sequence coding region. Gene 12: 235-241, 1980. PubMed: 6265317