Comments |
Restriction digests of the clone give the following sizes (kb): BglI--3.7, 1.7, 1.2, 0.6; EcoRI/HindIII--4.5, 2.7; PstI--3.6, 1.6 (doublet), 0.6; PvuII--2.4, 1.4, 1.3, 1.1; SacI--4.5, 2.7; SmaI--4.6, 1.9, 0.4. The insert contains the following restriction sites (nt from the 5' end): PvuII--123, 377, 791, 2040, 3052; PstI--632, 1184, 1269, 2747; SmaI--2034, 2420; DraI--3564. Contains part of intron 2, exons 3-6, introns 3-5, and 3' flanking sequences. |