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Construct size (kb): 4.349999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D0S1001E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens myeloid differentiation primary response
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.35
Cross references: DNA Seq. Acc.: M77995; EST: EST01580; Primer: M13 Reverse;
THC id: THC101105

Insert lengths(kb): 1.350000023841858

Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205)

Gene product: myeloid differentiation primary response(myeloid differentiation primary response gene MyD118) [Myd118*]

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.35; BamHI--4.35; PstI--4.35.

EST01580 matches GenPept accession MUSMYD118_1 with 88.31% identity and 94.80% similarity over the sequence range: 128-358.

Sequenced from the M13 reverse primer.

This sequence record is for mouse myeloid differentiation primary response.