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Construct size (kb): 7.5

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC
Intact vector size: 2.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: COL1A1
Genomic copy number: unique
Gene name: collagen, type I, alpha 1
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q21.3-q22
Tissue: fibroblast cell line SV80
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 5.2
Cross references: DNA Seq. Acc.: X07884
Nucleotides 1-1500 of the insert correspond to
nucleotides 1-1500 of X07884.

Insert lengths(kb): 5.199999809265137

Tissue: fibroblast cell line SV80

Gene product: collagen, type I, alpha 1 [COL1A1]

Restriction digests of the clone give the following sizes (kb): BamHI--5.7, 2.3; EcoRI--4.6, 2.7, 0.64; PvuII--2.5, 2.0, 1.5, 0.65, 0.5, 0.35; SacI--7.0, 0.52; XhoI--4.9, 2.4, 0.7.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.9; EcoRI--4.5; KpnI--0.2; NcoI--0.6, 0.7; PvuII--0.6, 0.85, 2.3, 4.3; SacI--1.0, 1.5; XhoI--0.8, 1.5.

To obtain a high yield, grow the culture in minimal media with casamino acids (M9), followed by chloramphenicol amplification to increase the plasmid copy number.