Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4, 1.15; EcoRI--5.4; HindIII--5.4; PstI--3.8, 1.5; BamHI--5.4. Temperature of hybridization should be 48C. Enzyme(s) not detecting polymorphism: TaqI, EcoRV, PvuII, HindIII, BglII. |