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pTR1
pTR1
規(guī)格:
貨期:
編號:B238560
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pTR1
商品貨號 B238560
Designations pTR1
Species Rattus norvegicus, rat
Depositors LM Matrisian
Vector
Construct size (kb): 6.0
Insert
DNA: cDNA
Insert lengths(kb): 1.700000047683716
Tissue: PyT21 cell line
Gene product: matrix metalloproteinase 10( stromelysin 2, transin-2, stromelysin 2, stromelysin 2 (transin-2)) [MMP3]
Insert Size (kb) 1.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
The insert contains the following restriction sites (in nucleotides from the 5' end of the published sequence): PstI--151; HindIII--749; BamHI--1305.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.7; KpnI--6.0; HindIII--5.1, 0.9; PstI--4.3, 0.85 (doublet); BamHI--4.75, 1.25.
pTR1 detects genomic EcoRI fragments of 6.0, 4.1, and 1.9 kb in rats and genomic EcoRI fragments of 12.0, 8.0, 3.5, and 2.7 kb in humans.
The insert extends from nucleotides 91 to 1771 of the cDNA. The coding sequences extend from nucleotides 57 to 1482. The insert has the complete 3' untranslated region.
A 525 bp PstI-KpnI fragment (nucleotides 155-680) of pTR1 detects a 7.9 kb genomic EcoRI fragment in rat cells which corresponds to the related gene, transin-2.
pTR1 detects a 1.9 kb mRNA in both rat and mouse tumors.
References

McDonnell SE, et al. Epidermal growth factor stimulation of stromelysin mRNA in rat fibroblasts requires induction of proto-oncogenes c-fos and c-jun and activation of protein kinase C. Mol. Cell. Biol. 10: 4284-4293, 1990. PubMed: 2115124

Matrisian LM, et al. Epidermal growth factor and oncogenes induce transcription of the same cellular mRNA in rat fibroblasts. EMBO J. 4: 1435-1440, 1985. PubMed: 3875482

Kerr LD, et al. Growth factors regulate transin gene expression by c-fos-dependent and c-fos-independent pathways. Science 242: 1424-1427, 1988. PubMed: 2462278

Kerr LD, et al. Transforming growth factor beta1 and cAMP inhibit transcription of epidermal growth factor- and oncogene-induced transin mRNA. J. Biol. Chem. 263: 16999-17005, 1988. PubMed: 2846555

Matrisian LM, et al. Differential expression of preproenkephalin and transin mRNAs following oncogenic transformation: evidence for two classes of oncogene induced genes. Oncogene Res. 2: 251-262, 1988. PubMed: 2835738

Matrisian LM, et al. The mRNA coding for the secreted protease transin is expressed more abundantly in malignant than benign tomors. Proc. Natl. Acad. Sci. USA 83: 9413-9417, 1986. PubMed: 3540938

Matrisian LM, et al. Isolation of the oncogene and epidermal growth factor-induced transin gene: complex control in rat fibroblasts. Mol. Cell. Biol. 6: 1679-1686, 1986. PubMed: 2431284

Breathnach R, et al. Sequences coding for part of oncogene-induced transin are highly conserved in a related rat gene. Nucleic Acids Res. 15: 1139-1151, 1987. PubMed: 3547333

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