Produces giant cells most effectively upon transfer/split of inoculated cells.
Produces giant cells most effectively upon transfer/split of inoculated cells.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Buffy coat cells from asymptomatic cat
Product Format
frozen
Storage Conditions
-60°C
Comments
Produces giant cells most effectively upon transfer/split of inoculated cells. Syncytia formation more dramatic in fibroblastic cells rather than CRFK. Freeze-labile when not frozen with 10% DMSO.
Effect on Host
Cytopathic effects (syncytium formation and sloughing)
Recommended Host
CRFK cells (ATCC CCL-94)
feline cell cultures
Growth Conditions
Temperature: 37°C
Incubation: 7 days
Name of Depositor
JM Gaskin
Source
Buffy coat cells from asymptomatic cat
References
Riggs JL, et al. Syncytium-forming agent isolated from domestic cats. Nature 222: 1190-1191, 1969. PubMed: 5788991