| 產(chǎn)品名稱 |
pCFH |
| 商品貨號(hào) |
B163206 |
| Designations |
pCFH |
| Species |
Beet curly top virus |
| Depositors |
DC Stenger |
| Applications |
Contains sequence Plant research |
| Vector |
Construct size (kb): 5.99
Construction of the clone: Plasmid constructed by inserting 2927 bp EcoRI linearized viral dsDNA into EcoRI site of pUC8vector. |
| Insert |
DNA: cDNA
Insert lengths(kb): 2.927
Tissue: beet severe curly top virus |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Plasmid constructed by inserting 2927 bp EcoRI linearized viral dsDNA into EcoRI site of pUC8 vector. The clone is infectious. ATCC sample contains 50 µg of purified plasmid DNA in 50 µL TE buffer. |
| Classification |
Curtovirus |
| References |
Stenger DC. Complete nucleotide sequence of the hypervirulent CFH strain of beet curly top virus. Mol. Plant-Microbe Interact. 7: 154-157, 1994. PubMed: 8167369
|
