產(chǎn)品名稱(chēng) |
Human rhinovirus 50 |
商品貨號(hào) |
B162830 |
Classification |
Picornaviridae, Enterovirus, Human rhinovirus A |
Deposited As |
Rhinovirus 50 |
Agent |
Human rhinovirus 50 |
Strain |
A2#58 |
Applications |
Respiratory research |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1.0 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Comments |
ATCC® VR-1876™ was derived from ATCC® VR-517™ following treatment with diethyl ether to remove mycoplasma contamination. |
Effect on Host |
CPE, refractile rounding and sloughing |
Recommended Host |
HeLa cells (ATCC® CCL-2™) |
Growth Conditions |
Temperature: 33°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1.5-2.0 hours at 33°C in a humidified 5% CO2 atmosphere while rocking. End adsorption by adding virus growth medium.
Incubation: 6-7 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
Name of Depositor |
VV Hamparian, RM Conant |
Chain of Custody |
ATCC <-- VV Hamparian, RM Conant |
Source |
Human throat washings |