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Human echovirus 9
Human echovirus 9
規(guī)格:
貨期:
編號(hào):B162821
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Human echovirus 9
商品貨號(hào) B162821
Classification Picornaviridae, Enterovirus, Enterovirus B
Deposited As Echovirus 9
Agent Human echovirus 9
Strain Vispo
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1888 images
Comments Derived by adaptation of primary cell culture product ATCC® VR-1051™ to LLC-MK2 Derivative cells (ATCC® CCL-7.1™). ATCC® VR-1888™ and ATCC® VR-1051™ have not been compared for sequence or infectivity in primary cell culture and tissue culture.

The original source material has been reported to contain Simian virus 40.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
LLC-MK2 Derivative cells (ATCC® CCL-7.1™)
Growth Conditions

Temperature: 36°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 2 hours at 36°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 3-4 days at 36°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer.

Name of Depositor NIH/NIAID
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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