Vector |
Construct size (kb): 6.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCR3 Intact vector size: 5.100 Type of vector: phagemid Vector end: TA site Vector end: TA site Cloning sites: TA cloning between EcoRI sites Polylinker sites: HindIII KpnI SacI BamHI SpeI XmaIII BstXI EcoRI EcoRI PstI EcoRV BstXI NotI XhoI XbaI ApaI Construction: pcDNA3 Features (with orientation and position when available): promoter: T7, -> MCS: HindIII/.../TA cloning site/.../ApaI, -> promoter: SP6, <- other: bovine growth hormone polyA signal, -> replicon: pMB1 marker(s): kanR, <- promoter: SV40, <- replicon: SV40, <- marker(s): ampR, <- replicon: f1, -> promoter: cytomegalovirus, -> Cross references: |
Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--7.0; EcoRI--5.6, 1.5; XhoI--5.6, 0.82; NotI--7.0; SacI--5.2, 1.1, 0.52, 0.084. The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.46; XhoI--0.65. |