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p171
p171
規(guī)格:
貨期:
編號:B161893
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p171
商品貨號 B161893
Designations p171
GenBank Number

K01691

Species Moloney murine sarcoma virus, Moloney murine sarcoma virus
Depositors UA Rapp
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 0.699999988079071
Gene product: murine leukemia viral (v-raf-1) oncogene homolog 1( Craf1 transforming gene, murine sarcoma 3611 oncogene 1, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1, Oncogene RAF1) [RAF1]
Alleles: B, M, B1, A1, A2, B2
Insert Size (kb) 0.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): AvaI--4.38; EcoRI/PvuII--2.3, 1.8; NruI--4.2; EcoRI/NruI--3.4, 0.84.
The insert contains AvaI, AvaII, StuI(2), HpaI, and SphI sites.
Constructed by inserting an XhoI/SstII fragment into vector from which the HindIII/SalI fragment had been removed. The SstIII and HindIII sites were blunt-ended before ligation.
Classification Retroviridae, Gammaretrovirus, Mammalian virus group
References

Mark GE, Rapp UR. Primary structure of v-raf: relatedness to the src family of oncogenes. Science 224: 285-289, 1984. PubMed: 6324342

Rapp UR, et al. Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc. Natl. Acad. Sci. USA 80: 4218-4222, 1983. PubMed: 6308607

Kozak C, et al. A new oncogene, c-raf, is located on mouse chromosome 6. J. Virol. 49: 297-299, 1984. PubMed: 6690719

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